The fractionation of phosphate esters on ion-exchange resin by a new system of pH-gradient elution.
نویسنده
چکیده
Chargaff, E. & Cohen, S. S. (1939). J. biol. Chem. 129, 619. Dawson, R. M. C. & Elliott, W. H. (1959). In Data for Biochemical Re8earch, p. 192. Ed. by Dawson, R. M. C., Elliott, D. C., Elliott, W. H. & Jones, K. M. Oxford: Clarendon Press. Fairbairn, D. (1945). J. biol. Chem. 157, 633. Folch, J., Lees, M. & Sloane-Stanley, G. H. (1957). J. biol. Chem. 226, 497. Gomori, G. (1955). In Methods in Enzymology, vol. 1, p. 143. Ed. by Colowick, S. P. & Kaplan, N. 0. New York: Academic Press Inc. Habermann, E. (1957). Biochem. Z. 328, 474. Hanahan, D. J. (1952). J. biol. Chem. 195, 199. Hanahan, D. J., Rodbell, M. & Turner, L. D. (1954). J. biol. Chem. 206, 431. Hayaishi, 0. & Kornberg, A. (1954). J. biol. Chem. 206,647. Hughes, A. (1935). Biochem. J. 29, 437. Kates, M. & Gorham, P. R. (1957). Canad. J. Biochem. Phy8iol. 35, 119. Laidler, K. J. (1955). Tranm. Faraday Soc. 51, 550. Levene, P. A. & Rolf, I. P. (1923). J. biol. Chem. 55, 743. Levene, P. A., Rolf, I. P. & Simms, H. S. (1924). J. biol. Chem. 58, 859. Lineweaver, H. & Burk, D. (1934). J. Amer. chem. Soc. 56, 658. Long, C. & Penny, I. F. (1957). Biochem. J. 65, 382. Rapport, M. M. & Franzl, R. E. (1957). J. biol. Chem. 225, 851. Rezek, A. (1945). Vet. Arhiv, 15, 161. Rhodes, D. N. & Lea, C. H. (1957). Biochem. J. 65, 526. Rimon, A. & Shapiro, B. (1959). Biochem. J. 71, 620. Stern, I. & Shapiro, B. (1953). Brit. J. clin. Path. 6, 158. Zamecnik, P. C., Brewster, L. E. & Lipmann, F. (1947). J. exp. Med. 85, 381. Zeller, E. A. (1950). In The Enzymne8, vol. 1, p. 988. Ed. by Sumner, J. B. & Myrback, K. New York: Academic Press Inc.
منابع مشابه
Synthesis of neopentyl glycol and ethylene glycol esters by fatty acids in the presence of acidic ion exchange resin catalyst
A most effective and less energy demanding method of producing fatty esters, diol esters, by esterifying fatty acids, with neopentyl and ethylenglycol alcohols in the presence of an acidic ion exchange resin catalyst(polyestyrendivinylbenzensulfated) was investigated at elevated temperature. In this process an azeotroping agent, toluene, was used to facilitate continuous removal of water by dis...
متن کاملImmunoglobulin A Preparation from Human Pooled Plasma Using Plasma Fractionation and Ion Exchange Chromatography
Background: In this study we have prepared the IgA solution from normal human plasma using plasma fractionation and ion exchange chromatography. Materials and Methods: Using fractionation of plasma with cold ethanol (starting with 8% ethanol), fraction III was prepared as a suitable source for IgA preparation. Then it was treated with caprylic acid for separation of impurities. For enrichm...
متن کاملIon Exchange Column Performance Model for Separation of Zr(IV) and Hf(IV) in Elution Process
A mathematical model of sorption of Zirconium and Hafnium cations mixture and elution of these ions from a cation exchange resin bed is developed. Sulfuric acid is selected as the eluent for selective separation of Zr and Hf in a packed bed of Dowex cation exchange resin. The column model is a dispersed plug flow model,and assumed to consist of randomly packed synthetic and spherical resin ...
متن کاملChromatography of ribonuclease on carboxymethyl cellulose columns.
In the course of an investigation of the chemical phosphorylation of ribonucleasel (cf. (l)), it became necessary to develop a chromatographic procedure for the fractionation of the phosphorylated protein. The method of Hirs et al. (2), employing the weak cation exchanger Amberlite XE-64, is an extremely valuable method for the chromatographic purification of pancreatic ribonuclease, but the us...
متن کاملBiocompatibility research of a novel pH sensitive ion exchange resin microsphere
The main objective of this study was to investigate biocompatibility and provide in vivo pharmacological and toxicological evidence for further investigation of the possibility of pH sensitive ion exchange resin microsphere for clinical utilizations. Acute toxicity study and general pharmacological studies were conducted on the pH sensitive ion exchange resin microsphere we prepared. The genera...
متن کاملBiocompatibility research of a novel pH sensitive ion exchange resin microsphere
The main objective of this study was to investigate biocompatibility and provide in vivo pharmacological and toxicological evidence for further investigation of the possibility of pH sensitive ion exchange resin microsphere for clinical utilizations. Acute toxicity study and general pharmacological studies were conducted on the pH sensitive ion exchange resin microsphere we prepared. The genera...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- The Biochemical journal
دوره 77 شماره
صفحات -
تاریخ انتشار 1960